The most common 2D experiments on the automated systems work best if you choose the one whose names end in “-opt”; these experiments examine a preliminary PROTON8 experiment and reset the 1H spectrum window in the 2D so you get the best resolution. It’s very easy to set up, but things can go wrong if you make changes or decide you need a 2D after the 1H 1D has already been acquired. Here are some tips for success.
Many chemical structure questions can be answered with 1H-1H distance estimates derived from the 1D NOESY experiment. There are a variety of ways to set up this experiment, but we now have two short videos that UChicago users can access with their phones at the spectrometer – no homework or special prep required.
The new 400-2 is ready for use! With this upgrade, the spectrometer has transformed into an automated high-throughput engine for routine data acquisition. “Routine” now includes a variety of standard 2D experiments, plus 1D experiments for a growing assortment of NMR-active nuclei.
Major events took place in Week 1 of reopening, including a number of hardware and software upgrades to NMR systems. For Week 2, expect to continue using 400-1 for routine work. Other systems are actively being configured and upgraded in various ways, so please be patient.